Daniel:Protocols/Qiaquick Column: Difference between revisions
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>Djacobse (Created page with "=Qiaquick Column Purification= Qiagen: Catalog #28104 (50) or #28106 (250) ==Protocol== 1. Add 5X PB (binding buffer) to sample 2. Load sample onto column and spin for 1...") |
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=Qiaquick Column Purification= | =Qiaquick Column Purification= | ||
Qiagen: Catalog #28104 (50) or #28106 (250) | [[Daniel Jacobsen|Back to Main]] | ||
'''Qiagen: Catalog #28104 (50) or #28106 (250)''' | |||
==Protocol== | ==Protocol== | ||
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8. Let stand 1 minute | 8. Let stand 1 minute | ||
9. Spin for 1 minute at 14000 rpm | 9. Spin for 1 minute at 14000 rpm | ||
Revision as of 22:40, 31 July 2013
Qiaquick Column Purification
Qiagen: Catalog #28104 (50) or #28106 (250)
Protocol
1. Add 5X PB (binding buffer) to sample 2. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through 3. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through 4. Dry spin column for 1 minute at 14000 rpm; discard flow through 5. Let stand with cover open in fume hood for ~5 minutes 6. Transfer column to a new 1.5 mL eppendorf tube 7. Add ≥30 uL EB to column 8. Let stand 1 minute 9. Spin for 1 minute at 14000 rpm