Daniel:Protocols/Qiaquick Column: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
No edit summary
>Djacobse
 
Line 6: Line 6:


==Protocol==
==Protocol==
  1. Add 5X PB (binding buffer) to sample
 
  2. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
<ol>
  3. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
<li>Add 5X PB (binding buffer) to sample</li>
  4. Dry spin column for 1 minute at 14000 rpm; discard flow through
<li>Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through</li>
  5. Let stand with cover open in fume hood for ~5 minutes
<li>Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through</li>
  6. Transfer column to a new 1.5 mL eppendorf tube
<li>Dry spin column for 1 minute at 14000 rpm; discard flow through</li>
  7. Add ≥30 uL EB to column
<li>Let stand with cover open in fume hood for ~5 minutes</li>
  8. Let stand 1 minute
<li>Transfer column to a new 1.5 mL eppendorf tube</li>
  9. Spin for 1 minute at 14000 rpm
<li>Add ≥30 uL EB to column</li>
<li>Let stand 1 minute</li>
<li>Spin for 1 minute at 14000 rpm</li>
</ol>

Latest revision as of 16:40, 28 February 2017

Qiaquick Column Purification[edit]

Back to Main

Qiagen: Catalog #28104 (50) or #28106 (250)

Protocol[edit]

  1. Add 5X PB (binding buffer) to sample
  2. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
  3. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
  4. Dry spin column for 1 minute at 14000 rpm; discard flow through
  5. Let stand with cover open in fume hood for ~5 minutes
  6. Transfer column to a new 1.5 mL eppendorf tube
  7. Add ≥30 uL EB to column
  8. Let stand 1 minute
  9. Spin for 1 minute at 14000 rpm