Blue:RNA-Seq Experiments:04052013: Difference between revisions

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Purpose:  Test the effects of lysis buffer on RNAseIII fragmentation-based single cell RNA-seq protocol
Results:  Tested water or RNaseIII buffer with or without Triton-x100 (0.1%, 0.2%), NP-40 (0.1%, 0.2%, 0.5%) or RNase Inhibitor.  Best condition: RNaseIII buffer + 0.1% Triton X-100 and No RNase Inhibitor
[[File:04052013.pdf]]
[[File:04052013.pdf]]

Latest revision as of 00:59, 19 April 2013

Purpose: Test the effects of lysis buffer on RNAseIII fragmentation-based single cell RNA-seq protocol

Results: Tested water or RNaseIII buffer with or without Triton-x100 (0.1%, 0.2%), NP-40 (0.1%, 0.2%, 0.5%) or RNase Inhibitor. Best condition: RNaseIII buffer + 0.1% Triton X-100 and No RNase Inhibitor


File:04052013.pdf