Hosuk:LabNotes/2013-4-23: Difference between revisions

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>Hosuki78
(Created page with "====RCA test with the product of CircLigase II reaction==== =====Goal===== *Verify that there are circularized DNA in the CircLigase II reaction sample =====Experiment Proce...")
 
>Hosuki78
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=====Experiment Procedure=====
=====Experiment Procedure=====
*Run RCA
*Run RCA
**S1 : CircLigase II reaction sample (18hr. @ 30°C)
**S2 : -CircLigase II sample (No CircLigase II Enzyme while CircLigase II reaction) (18hr. @ 30°C)
**Protocol
**Protocol
[[File:RCAReationProtocolinTube.jpg|400px]]
**[[File:RCAReationProtocolinTube.jpg|350px]]
 
*Mixing Cy3-Adapter
**4.5uL of each RCA sample + 0.5uL of 100uM Cy3-Adapter
**S1 : +CircLigase II + Cy3-Adapter
**S2 : -CircLigase II + Cy3-Adapter
**S3 : H2O + Cy3-Adapter

Revision as of 00:20, 24 April 2013

RCA test with the product of CircLigase II reaction

Goal
  • Verify that there are circularized DNA in the CircLigase II reaction sample


Experiment Procedure
  • Run RCA
    • S1 : CircLigase II reaction sample (18hr. @ 30°C)
    • S2 : -CircLigase II sample (No CircLigase II Enzyme while CircLigase II reaction) (18hr. @ 30°C)
    • Protocol
    • File:RCAReationProtocolinTube.jpg
  • Mixing Cy3-Adapter
    • 4.5uL of each RCA sample + 0.5uL of 100uM Cy3-Adapter
    • S1 : +CircLigase II + Cy3-Adapter
    • S2 : -CircLigase II + Cy3-Adapter
    • S3 : H2O + Cy3-Adapter