Daniel:Reagents/LambdaExonuclease: Difference between revisions

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Lambda Exonuclease datasheet (NEB): [[File:M0262-lambda-exonuclease.pdf]]
Lambda Exonuclease datasheet (NEB): [[File:M0262-lambda-exonuclease.pdf]]


  *1 unit is defined as the amount of
  *1 unit is defined as the amount of enzyme required to produce 10 nmol of deoxyribonucleotide from double-stranded
substrate in a total reaction volume of 50 uL in 30 minutes at 37C in 1X reaction buffer with 1 ug of H3-DNA (NEB)
 
Heat Shock
Heat inactivate for 10 minutes at 75 C.


NEB
NEB

Latest revision as of 16:36, 3 May 2013

Lambda Exonuclease[edit]

Lambda Exonuclease datasheet (NEB): File:M0262-lambda-exonuclease.pdf

*1 unit is defined as the amount of enzyme required to produce 10 nmol of deoxyribonucleotide from double-stranded
substrate in a total reaction volume of 50 uL in 30 minutes at 37C in 1X reaction buffer with 1 ug of H3-DNA (NEB)

Heat Shock

Heat inactivate for 10 minutes at 75 C.

NEB

M0262S	1,000 units  5,000 units/ml  $63.00
M0262L	5,000 units  5,000 units/ml  $252.00