Hosuk:LabNotes/2013-5-2: Difference between revisions

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>Hosuki78
(Created page with "====Result - RT check==== *based on Debugging plan (05/01) *+RTase sample showed very strong Cy5 signal which is supposed cDNA *comparing to -RTas...")
 
>Hosuki78
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*not many spots were observed, which spots were outside of cells.
*not many spots were observed, which spots were outside of cells.


*'''+RTase'''
{| {{table}}
{| {{table}}
| S2:+RTase, Brighfield, 20x
| S2:+RTase, Brighfield, 20x
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||
||
|[[File:Composite_+RTase_fig07-08.jpg|300px]]
|[[File:Composite_+RTase_fig07-08.jpg|300px]]
|}
*'''-RTase'''
{| {{table}}
| S3:-RTase, Brighfield, 20x
||
| S3:-RTase, Cy5, 20x
||
| S3:-RTase, Merged
||
|-
|([[Media:RTCheckNoRTase_fig08_20xobj_pos05_BF_exp0.2-EM00.tif|Original]])
||
|([[Media:RTCheckNoRTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.tif|Original]])
||
|
|-
|[[File:RTCheckNoRTase_fig08_20xobj_pos05_BF_exp0.2-EM00.jpg|300px]]
||
|[[File:RTCheckNoRTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.jpg|300px]]
||
|[[File:Composite_NoRTase_fig07-08.jpg|300px]]
|}
|}

Revision as of 22:34, 2 May 2013

Result - RT check

  • based on Debugging plan (05/01)
  • +RTase sample showed very strong Cy5 signal which is supposed cDNA
  • comparing to -RTase result, the bright singal over the cell seemed they were cDNAs
  • Most of signal came from cell structure.
  • However I don't know what are the large spots. Are they also cDNAs?
  • -RTase sample has very low intensity of signals in the cell, and
  • not many spots were observed, which spots were outside of cells.
  • +RTase
S2:+RTase, Brighfield, 20x S2:+RTase, Cy5, 20x S2:+RTase, Merged
(Original) (Original)
File:RTCheck +RTase fig08 20xobj pos04 BF exp0.2-EM00.jpg File:RTCheck +RTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg File:Composite +RTase fig07-08.jpg


  • -RTase
S3:-RTase, Brighfield, 20x S3:-RTase, Cy5, 20x S3:-RTase, Merged
(Original) (Original)
File:RTCheckNoRTase fig08 20xobj pos05 BF exp0.2-EM00.jpg File:RTCheckNoRTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg File:Composite NoRTase fig07-08.jpg