Daniel:Notebook/HiResChrPaint/2013-5-4: Difference between revisions
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=Probe Prep Test ([[Daniel:Notebook/HiResChrPaint/2013-4-22|Started 4/22/2013]])= | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | |||
==Ethanol Precipitation (Continued from [[Daniel:Notebook/HiResChrPaint/2013-5-3|5/3/2013]]== | |||
3. Spin at 12000 rpm for 30 minutes at 4 C | |||
4. Remove supernatant and add 750 uL chilled 75% EtOH | |||
5. Centrifuge at 12000 rpm for 15 minutes at 4 C | |||
6. Remove supernatant and dry in hood | |||
7. Resuspend in 7 uL nuclease free H20 | |||
==TBU gel== | |||
Revision as of 17:06, 7 May 2013
Probe Prep Test (Started 4/22/2013)
Ethanol Precipitation (Continued from 5/3/2013
3. Spin at 12000 rpm for 30 minutes at 4 C 4. Remove supernatant and add 750 uL chilled 75% EtOH 5. Centrifuge at 12000 rpm for 15 minutes at 4 C 6. Remove supernatant and dry in hood 7. Resuspend in 7 uL nuclease free H20