Daniel:Notebook/HiResChrPaint/2013-5-4: Difference between revisions

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=Probe Prep Test ([[Daniel:Notebook/HiResChrPaint/2013-4-22|Started 4/22/2013]])=


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
==Ethanol Precipitation (Continued from [[Daniel:Notebook/HiResChrPaint/2013-5-3|5/3/2013]]==
3. Spin at 12000 rpm for 30 minutes at 4 C
4. Remove supernatant and add 750 uL chilled 75% EtOH
5. Centrifuge at 12000 rpm for 15 minutes at 4 C
6. Remove supernatant and dry in hood
7. Resuspend in 7 uL nuclease free H20
==TBU gel==


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]





Revision as of 17:06, 7 May 2013

Probe Prep Test (Started 4/22/2013)

Back to Calendar

Ethanol Precipitation (Continued from 5/3/2013

3. Spin at 12000 rpm for 30 minutes at 4 C
4. Remove supernatant and add 750 uL chilled 75% EtOH
5. Centrifuge at 12000 rpm for 15 minutes at 4 C
6. Remove supernatant and dry in hood
7. Resuspend in 7 uL nuclease free H20

TBU gel

A File:LamdaDigest 050413A.jpg

B File:LamdaDigest 050413B.jpg