Daniel:Notebook/HiResChrPaint/2013-5-8: Difference between revisions
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[[Daniel:Protocols/TBUgel|Protocol 2]] | [[Daniel:Protocols/TBUgel|Protocol 2]] | ||
1. Gel lanes | 1. Gel lanes | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt" align="center" | |||
| width="65" height="39" | Sample | |||
| width="50" | 0.5 Ladder | |||
| width="50" | 1 uL Ladder | |||
| width="50" | Original | |||
| width="50" | Column (1uL) | |||
| width="50" | Column (2uL) | |||
| width="50" | EtOH (1 uL) A | |||
| width="50" | EtOH (2uL) A | |||
| width="50" | EtOH (1 uL) B | |||
| width="50" | EtOH (2uL) B | |||
|- style="font-size:12pt" align="center" | |||
| height="15" | uL sample | |||
| align="center" | 0.5 | |||
| align="center" | 1 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
|- style="font-size:12pt" align="center" | |||
| height="15" | uL TBE | |||
| align="center" | 9.5 | |||
| align="center" | 9 | |||
| align="center" | 9.5 | |||
| align="center" | 9.5 | |||
| align="center" | 9.5 | |||
| align="center" | 9.5 | |||
| align="center" | 9.5 | |||
| align="center" | 9.5 | |||
| align="center" | 9.5 | |||
|- style="font-size:12pt" align="center" | |||
| height="15" | uL 2X buffer | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
| align="center" | 10 | |||
|- style="font-size:12pt" align="center" | |||
| height="15" | Loaded | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
| align="center" | 4 | |||
|- style="font-size:12pt" align="center" | |||
| height="15" | Dilution | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
| align="center" | 0.1 | |||
|} |
Revision as of 22:28, 8 May 2013
Probe Prep Test (Started 4/22/2013)
Column Purification vs. Ethanol Precipitation
Ethanol Precipitation (continued from 5/6/2013)
5. Centrifuged 30 min at 10000 rpm at 4C 6. Removed supernatant and added 750 uL chilled EtOH 7. Centrifuged 15 min at 10000 rpm at 4C 8. Removed supernatant and dried in hood 9. Resuspended in 10 uL nf H20
TBU Gel comparison
1. Gel lanes
Sample | 0.5 Ladder | 1 uL Ladder | Original | Column (1uL) | Column (2uL) | EtOH (1 uL) A | EtOH (2uL) A | EtOH (1 uL) B | EtOH (2uL) B |
uL sample | 0.5 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
uL TBE | 9.5 | 9 | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 |
uL 2X buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
Loaded | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
Dilution | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |