Daniel:Notebook/HiResChrPaint/2013-5-8: Difference between revisions

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[[Daniel:Protocols/TBUgel|Protocol 2]]
[[Daniel:Protocols/TBUgel|Protocol 2]]
  1. Gel lanes
  1. Gel lanes
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center"
| width="65" height="39" | Sample
| width="50" | 0.5 Ladder
| width="50" | 1 uL Ladder
| width="50" | Original
| width="50" | Column (1uL)
| width="50" | Column (2uL)
| width="50" | EtOH    (1 uL) A
| width="50" | EtOH (2uL) A
| width="50" | EtOH    (1 uL) B
| width="50" | EtOH (2uL) B
|- style="font-size:12pt" align="center"
| height="15" | uL sample
| align="center" | 0.5
| align="center" | 1
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
|- style="font-size:12pt" align="center"
| height="15" | uL TBE
| align="center" | 9.5
| align="center" | 9
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
|- style="font-size:12pt" align="center"
| height="15" | uL 2X buffer
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
|- style="font-size:12pt" align="center"
| height="15" | Loaded
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
|- style="font-size:12pt" align="center"
| height="15" | Dilution
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
| align="center" | 0.1
|}

Revision as of 22:28, 8 May 2013

Probe Prep Test (Started 4/22/2013)

Back to Calendar

Column Purification vs. Ethanol Precipitation

Ethanol Precipitation (continued from 5/6/2013)

5. Centrifuged 30 min at 10000 rpm at 4C
6. Removed supernatant and added 750 uL chilled EtOH
7. Centrifuged 15 min at 10000 rpm at 4C
8. Removed supernatant and dried in hood
9. Resuspended in 10 uL nf H20

TBU Gel comparison

Protocol 2

1. Gel lanes
Sample 0.5 Ladder 1 uL Ladder Original Column (1uL) Column (2uL) EtOH (1 uL) A EtOH (2uL) A EtOH (1 uL) B EtOH (2uL) B
uL sample 0.5 1 0.5 0.5 0.5 0.5 0.5 0.5 0.5
uL TBE 9.5 9 9.5 9.5 9.5 9.5 9.5 9.5 9.5
uL 2X buffer 10 10 10 10 10 10 10 10 10
Loaded 4 4 4 4 4 4 4 4 4
Dilution 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1