Daniel:Notebook/HiResChrPaint/2013-5-11: Difference between revisions

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==DpnII Digestion==
==DpnII Digestion==


[[Daniel:Reagents/DpnII|Info]]
[[Daniel:Reagents/DpnII|Enzyme Info]]


  1. Prepared samples according to table
  1. Prepared samples according to table
  *DpnII-Lot 0091201; Buffer 0031105

Revision as of 02:30, 12 May 2013

Probe Prep Test 2 (Started 5/10/2013)

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Ethanol Precipitation

7. Centrifuged 10000 rpm for 30 minutes at 4C
8. Removed supernatant added 750 uL 75% EtOH
9. Centrifuged 10000 rpm for 12 minutes at 4C
10. Removed supernatant, dried in hood
11. Resuspended with 50 uL
12. Column Purification, eluted with 50 uL
13. measured concentration in nanodrop

Nanodrop Results

Sample V4A V4B V4C V4D V6A V6B V6C V6D
ng/uL 127.5 156.3 130.2 130.6 142.7 138.9 146.4 133.4
ng 5100 6252 5208 5224 5708 5556 5856 5336
ug in 4 sets       21.8 22.5

DpnII Digestion

Enzyme Info

1. Prepared samples according to table
  *DpnII-Lot 0091201; Buffer 0031105