Daniel:Notebook/HiResChrPaint/2013-5-11: Difference between revisions
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==DpnII Digestion== | ==DpnII Digestion== | ||
[[Daniel:Reagents/DpnII|Info]] | [[Daniel:Reagents/DpnII|Enzyme Info]] | ||
1. Prepared samples according to table | 1. Prepared samples according to table | ||
*DpnII-Lot 0091201; Buffer 0031105 |
Revision as of 02:30, 12 May 2013
Probe Prep Test 2 (Started 5/10/2013)
Ethanol Precipitation
7. Centrifuged 10000 rpm for 30 minutes at 4C 8. Removed supernatant added 750 uL 75% EtOH 9. Centrifuged 10000 rpm for 12 minutes at 4C 10. Removed supernatant, dried in hood 11. Resuspended with 50 uL 12. Column Purification, eluted with 50 uL 13. measured concentration in nanodrop
Nanodrop Results
Sample | V4A | V4B | V4C | V4D | V6A | V6B | V6C | V6D |
ng/uL | 127.5 | 156.3 | 130.2 | 130.6 | 142.7 | 138.9 | 146.4 | 133.4 |
ng | 5100 | 6252 | 5208 | 5224 | 5708 | 5556 | 5856 | 5336 |
ug in 4 sets | 21.8 | 22.5 |
DpnII Digestion
1. Prepared samples according to table *DpnII-Lot 0091201; Buffer 0031105