Daniel:Notebook/HiResChrPaint/2013-5-13: Difference between revisions
Jump to navigation
Jump to search
>Djacobse (Created page with "=Probe Prep Test 2 (Started 5/10/2013)= Back to Calendar ==TBE Gel== 1. 0.5 uL sample; 6 uL T...") |
>Djacobse No edit summary |
||
Line 6: | Line 6: | ||
1. 0.5 uL sample; 6 uL TBE, 2 uL dye per sample | 1. 0.5 uL sample; 6 uL TBE, 2 uL dye per sample | ||
2. Gel Arrangement: | 2. Gel Arrangement(5-12 are digested samples that have been column purified, ready to use for lambda exo digestion): | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |
Revision as of 17:47, 13 May 2013
Probe Prep Test 2 (Started 5/10/2013)
TBE Gel
1. 0.5 uL sample; 6 uL TBE, 2 uL dye per sample 2. Gel Arrangement(5-12 are digested samples that have been column purified, ready to use for lambda exo digestion):
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp Ladder | V6S3A Undigested | V4S3C Pre-Column | V6S3B Pre-Column | V4S3A | V4S3B | V4S3C | V4S3D | V6S3A | V6S3B | V6S3C | V6S3D |
3. Loaded 7 uL per sample 4. Ran gel for 30 minutes at 200V 5. Stained 2 minutes with 3 uL SYBR gold