Matt:LabNotes/2013-5-17: Difference between revisions
Jump to navigation
Jump to search
>Mzcai |
>Mzcai |
||
Line 39: | Line 39: | ||
|} | |} | ||
*Ran at 220V for 30min | *Ran the 3 gels at 220V for 30min | ||
*Cut out band below 200bp | |||
Gel 1: <br> | |||
[[File:2013-05-17_Gel1SizeSelection_20gap.jpg| 450px]] | |||
Gel 2: <br> | |||
[[File:2013-05-17_Gel2SizeSelection_20gap.jpg| 450px]] | |||
Gel 3: <br> | |||
[[File:2013-05-17_Gel3SizeSelection_20gap.jpg| 450px]] |
Revision as of 23:12, 17 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16
PAGE-Size Selection of 20 gap probes
- Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
- I will run 3 gels each with 60ul (~3.4 ug of DNA each)
Sample
Components | 1X Volume | 3X Volume |
Enzyme digested probes (~10ug/180ul) | 55 | 165 |
TBE-Urea Buffer 2X | 55 | 165 |
Water | 0 | 0 |
Total | 110 | 330 |
3 Low Mass DNA Ladders
Components | 3X Volume |
Low Mass DNA ladder (1 ug/ul) | 3 |
TBE-Urea Buffer 2X | 15 |
H2O | 12 |
Total | 30 |
- Ran the 3 gels at 220V for 30min
- Cut out band below 200bp
Gel 1:
File:2013-05-17 Gel1SizeSelection 20gap.jpg