Daniel:Protocols/AlexaDyeCouple: Difference between revisions
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==Dye Couples== | |||
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==ARES Protocol== | |||
ARES Manual from Invitrogen: [[File:ARESTM DNA Labeling Kits.pdf]] | |||
1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature | |||
2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer | |||
3. Denature samples for 5 minutes at 95C, then snap cool using ice box | |||
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds | |||
*dye cannot be saved for later use. Use immediately! | |||
5. Add 1 uL dye to sample | |||
6. Incubate in the dark for 1 hour | |||
7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample | |||
8. Recommend column purification after incubation | |||
==ULYSIS Protocol=== | |||
ARES Manual from Invitrogen: [[File]] |
Revision as of 18:47, 3 July 2013
Alexa Fluorophore Dye Coupling
Dye Couples
Probe | Alexa Dye |
V4S1 | Alexa-546 |
V4S2 | Alexa-488 |
V4S3 | Alexa-594 |
V4S4 | Alexa-647 |
V6S1 | Alexa-546 |
V6S2 | Alexa-488 |
V6S3 | Alexa-594 |
V6S4 | Alexa-647 |
ARES Protocol
ARES Manual from Invitrogen: File:ARESTM DNA Labeling Kits.pdf
1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature 2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer 3. Denature samples for 5 minutes at 95C, then snap cool using ice box 4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds *dye cannot be saved for later use. Use immediately! 5. Add 1 uL dye to sample 6. Incubate in the dark for 1 hour 7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample 8. Recommend column purification after incubation
ULYSIS Protocol=
ARES Manual from Invitrogen: File