Matt:LabNotes/2013-5-21: Difference between revisions
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===Ethanol Precipitation after PAGE size selection=== | ===Ethanol Precipitation after PAGE size selection=== | ||
*Spun 6 tubes in -80C at 10,000rpm at 4C for 30min | *Spun 6 tubes in -80C at 10,000rpm at 4C for 30min | ||
* | *Discard supernatant and add 650ul 75% EtOH to wash and spin at 14,000rpm at 4C for 5 min | ||
*Discard supernatant and let dry in hood 10 min | |||
*Resuspended each of the 6 tubes with 10ul H2O (60ul total) | |||
===TBU Gel Quantification Plan=== | |||
*Dilute 2ul into 20ul | |||
*Mix 1:1 with 2X buffer (20X dilution) | |||
*Load wells with 2, 4, 6, 8 ul | |||
Continued on: |
Revision as of 01:13, 22 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-17
Ethanol Precipitation after PAGE size selection
- Spun 6 tubes in -80C at 10,000rpm at 4C for 30min
- Discard supernatant and add 650ul 75% EtOH to wash and spin at 14,000rpm at 4C for 5 min
- Discard supernatant and let dry in hood 10 min
- Resuspended each of the 6 tubes with 10ul H2O (60ul total)
TBU Gel Quantification Plan
- Dilute 2ul into 20ul
- Mix 1:1 with 2X buffer (20X dilution)
- Load wells with 2, 4, 6, 8 ul
Continued on: