Matt:LabNotes/2013-5-21: Difference between revisions

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===Ethanol Precipitation after PAGE size selection===
===Ethanol Precipitation after PAGE size selection===
*Spun 6 tubes in -80C at 10,000rpm at 4C for 30min
*Spun 6 tubes in -80C at 10,000rpm at 4C for 30min
*
*Discard supernatant and add 650ul 75% EtOH to wash and spin at 14,000rpm at 4C for 5 min
*Discard supernatant and let dry in hood 10 min
*Resuspended each of the 6 tubes with 10ul H2O (60ul total)
 
===TBU Gel Quantification Plan===
*Dilute 2ul into 20ul
*Mix 1:1 with 2X buffer (20X dilution)
*Load wells with 2, 4, 6, 8 ul
 
Continued on:

Revision as of 01:13, 22 May 2013

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-17

Ethanol Precipitation after PAGE size selection

  • Spun 6 tubes in -80C at 10,000rpm at 4C for 30min
  • Discard supernatant and add 650ul 75% EtOH to wash and spin at 14,000rpm at 4C for 5 min
  • Discard supernatant and let dry in hood 10 min
  • Resuspended each of the 6 tubes with 10ul H2O (60ul total)

TBU Gel Quantification Plan

  • Dilute 2ul into 20ul
  • Mix 1:1 with 2X buffer (20X dilution)
  • Load wells with 2, 4, 6, 8 ul

Continued on: