Matt:LabNotes/2013-5-29: Difference between revisions
Jump to navigation
Jump to search
>Mzcai |
>Mzcai |
||
Line 5: | Line 5: | ||
*0 gap: 11.36ug (28.4ng/ul) ~410ul | *0 gap: 11.36ug (28.4ng/ul) ~410ul | ||
* Ran TBU gel with samples and ladder prepared below | * Ran TBU gel at 220V for 30 min with samples and ladder prepared below | ||
** Heated TBE buffer in microwave | |||
** Denatured samples/ladder for 10 min at 75C before putting on cold rack for 1 min | |||
** Size selected for brightest band below 200bp | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Components''' | | align="center" style="background:#f0f0f0;"|'''Components''' | ||
Line 38: | Line 41: | ||
|} | |} | ||
Gel 1: <br> [[File:2013-05-29_Gel1SizeSelection_20gap.jpg | 450px]] | Gel 1: <br> [[File:2013-05-29_Gel1SizeSelection_20gap.jpg | 450px]] <br> | ||
Gel 2: <br> [[File:2013-05-29_Gel2SizeSelection_20gap.jpg | 450px]] | Gel 2: <br> [[File:2013-05-29_Gel2SizeSelection_20gap.jpg | 450px]] <br> | ||
Gel 3: <br> [[File:2013-05-29_Gel3SizeSelection_20gap.jpg | 450px]] | Gel 3: <br> [[File:2013-05-29_Gel3SizeSelection_20gap.jpg | 450px]] <br> | ||
Gel 4: <br> [[File:2013-05-29_Gel4SizeSelection_20gap.jpg | 450px]] | Gel 4: <br> [[File:2013-05-29_Gel4SizeSelection_20gap.jpg | 450px]] <br> |
Revision as of 22:33, 29 May 2013
Page Size Selection
- Noi prepared some 0 gap and 20 gap probes
*20 gap: 12.6ug (31.7ng/ul) ~410ul *0 gap: 11.36ug (28.4ng/ul) ~410ul
- Ran TBU gel at 220V for 30 min with samples and ladder prepared below
- Heated TBE buffer in microwave
- Denatured samples/ladder for 10 min at 75C before putting on cold rack for 1 min
- Size selected for brightest band below 200bp
Components | 1X Volume | 4X Volume |
Enzyme digested probes (~12.6ug/400ul) | 105 | 420 |
TBE-Urea Buffer 2X | 105 | 420 |
H2O | 0 | 0 |
Total | 210 | 840 |
Components | 1X Volume | 4X Volume |
10-bp DNA ladder (1 ug/ul) | 1 | 4 |
TBE-Urea Buffer 2X | 5 | 20 |
H2O | 4 | 16 |
total | 10 | 40 |
Gel 1:
File:2013-05-29 Gel1SizeSelection 20gap.jpg
Gel 2:
File:2013-05-29 Gel2SizeSelection 20gap.jpg
Gel 3:
File:2013-05-29 Gel3SizeSelection 20gap.jpg
Gel 4:
File:2013-05-29 Gel4SizeSelection 20gap.jpg