Matt:LabNotes/2013-5-31: Difference between revisions
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>Mzcai |
>Mzcai m (→Probe Capture) |
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===Probe Capture=== | ===Probe Capture=== | ||
*Use the ~ | *Use the ~37.4 pmole of probes from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22] and [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-29] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments | ||
*Use gDNA as template/target | *Use gDNA as template/target | ||
*Use a probe:target ratio of 100:1 | |||
====Probe:Target==== | ====100:1 Probe:Target Calculations==== | ||
{| {{table}} | |||
| Total Probes||3.74E-11||mole | |||
|- | |||
| Probes||2.88491E-15||mole (Total Probe/12,964 20 gap probes) | |||
|- | |||
| Target||2.88491E-17||mole (Probes/100) | |||
|- | |||
| Human gDNA MW||1.95E+12||g/mol (3x10^9 bp x 650 Da/bp + 157.9 Da) | |||
|- | |||
| Human gDNA mass||5.62558E-05||g (Target x Human gDNA MW) | |||
|- | |||
| | |||
|} |
Revision as of 20:39, 31 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22
Probe Capture
- Use the ~37.4 pmole of probes from [1] and [2] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
- Use gDNA as template/target
- Use a probe:target ratio of 100:1
100:1 Probe:Target Calculations
Total Probes | 3.74E-11 | mole |
Probes | 2.88491E-15 | mole (Total Probe/12,964 20 gap probes) |
Target | 2.88491E-17 | mole (Probes/100) |
Human gDNA MW | 1.95E+12 | g/mol (3x10^9 bp x 650 Da/bp + 157.9 Da) |
Human gDNA mass | 5.62558E-05 | g (Target x Human gDNA MW) |