Matt:LabNotes/2013-5-31: Difference between revisions

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Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22


===Probe Capture===
*Use the ~37.4 pmole of probes from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22] and [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-29] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
*Use gDNA as template/target
*Use a probe:target ratio of 100:1
====100:1 Probe:Target Calculations====
{| {{table}}
| Total Probes||3.74E-11||mole
|-
| Probes||2.88491E-15||mole (Total Probe/12,964 20 gap probes)
|-
| Target||2.88491E-17||mole (Probes/100)
|-
| Human gDNA MW||1.95E+12||g/mol (3x10^9 bp x 650 Da/bp + 157.9 Da)
|-
| Human gDNA mass||5.62558E-05||g (Target x Human gDNA MW)
|-
|
|}
56 ug of gDNA
140 ul of Probes

Latest revision as of 23:16, 4 June 2013