Matt:LabNotes/2013-6-12: Difference between revisions
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{| {{table}} border = 1 | {| {{table}} border = 1 | ||
| align="center" style="background:#f0f0f0;"|'''Components''' | | align="center" style="background:#f0f0f0;"|'''Components''' | ||
| align="center" style="background:#f0f0f0;"|'''Volume''' | | align="center" style="background:#f0f0f0;"|'''Volume (ul)''' | ||
|- | |- | ||
| Circle Oligos|| | | Circle Oligos||5 | ||
|- | |- | ||
| 10uM CP-2-FA||1 | | 10uM CP-2-FA||1 | ||
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| 2X KAPA SYBG||25 | | 2X KAPA SYBG||25 | ||
|- | |- | ||
| H2O|| | | H2O||18 | ||
|- | |- | ||
| Total||50 | | Total||50 |
Revision as of 21:05, 13 June 2013
Verify Probe Capture
Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'
Components | Volume (ul) |
Circle Oligos | 5 |
10uM CP-2-FA | 1 |
10uM CP-2-RA | 1 |
2X KAPA SYBG | 25 |
H2O | 18 |
Total | 50 |
Program 98C 30sec--> (98C 10sec --> 55C 30sec --> 72C 30sec)x8 cycles --> 72C 3 min --> 15C hold