Matt:LabNotes/2013-6-12: Difference between revisions

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{| {{table}} border = 1
{| {{table}} border = 1
| align="center" style="background:#f0f0f0;"|'''Components'''
| align="center" style="background:#f0f0f0;"|'''Components'''
| align="center" style="background:#f0f0f0;"|'''Volume'''
| align="center" style="background:#f0f0f0;"|'''Volume (ul)'''
|-
|-
| Circle Oligos||x
| Circle Oligos||5
|-
|-
| 10uM CP-2-FA||1
| 10uM CP-2-FA||1
Line 17: Line 17:
| 2X KAPA SYBG||25
| 2X KAPA SYBG||25
|-
|-
| H2O||x
| H2O||18
|-
|-
| Total||50
| Total||50

Revision as of 21:05, 13 June 2013

Verify Probe Capture

Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'

Components Volume (ul)
Circle Oligos 5
10uM CP-2-FA 1
10uM CP-2-RA 1
2X KAPA SYBG 25
H2O 18
Total 50
 Program
 98C 30sec--> (98C 10sec --> 55C 30sec --> 72C 30sec)x8 cycles --> 72C 3 min --> 15C hold