Hosuk:LabNotesMDA/2013-6-21: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 4th=== *..") |
>Hosuki78 No edit summary |
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===MDA with PGP1F, 4th=== | ===MDA with PGP1F, 4th=== | ||
* | *MDA run for 8hr | ||
*133mM ALS | |||
*Mixing pump --> shorter mixing time: 1sec period, 5min | |||
**Much less residue were seen under the valve area | |||
*Push ring mixer volume with air | |||
**Left 5~6 chamber were empty… why so many? | |||
*Verification of the amount of amplicon by qPCR : will be done soon | |||
*CoRE fragmentation was done @06/19 |
Revision as of 17:39, 21 June 2013
MDA with PGP1F, 4th
- MDA run for 8hr
- 133mM ALS
- Mixing pump --> shorter mixing time: 1sec period, 5min
- Much less residue were seen under the valve area
- Push ring mixer volume with air
- Left 5~6 chamber were empty… why so many?
- Verification of the amount of amplicon by qPCR : will be done soon
- CoRE fragmentation was done @06/19