Hosuk:LabNotesMDA/2013-6-28: Difference between revisions
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>Hosuki78 |
>Hosuki78 |
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*[[File:qPCR_Result_2013-06-28_Edited.png|800px]] | *[[File:qPCR_Result_2013-06-28_Edited.png|800px]] | ||
*[[File:qPCR_Result_MassTable_2013-06-28.png| | *[[File:qPCR_Result_MassTable_2013-06-28.png|200px]] | ||
====CoRE fragmentation==== | ====CoRE fragmentation==== | ||
*Done @06/28 | *Done @06/28 |
Revision as of 17:47, 28 June 2013
MDA with PGP1F, 6th
- MDA run for 8hr @06/27
- 400mM ALS --> original protocol
- Slow mixing --> 1 sec period, mixing for 5min
- Run MDA in ring mixer --> 25th chamber!
- Push ring mixer volume with air
- Left 8 chamber were empty, too many empty chamber...
Verification with qPCR
- Result looked good.
- Most of chambers have 500pg~2400pg of amplicons.
- Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.
CoRE fragmentation
- Done @06/28