Daniel:Notebook/HiResChrPaint/2013-7-8: Difference between revisions
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>Djacobse (Created page with "=S2 Probe Prep (Started 5/29/2013)= Back to Calendar ==ULYSIS Dye Coupling (Test)== Going to ...") |
>Djacobse No edit summary |
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1. Add 1 uL DNA in 19 uL labeling buffer (Component C) | 1. Add 1 uL DNA in 19 uL labeling buffer (Component C) | ||
2. Denature DNA for 5 minutes at 95C; snap cool on ice | |||
3. Add 1 uL dye to sample; add 4 uL labeling buffer | |||
4. Incubate for 15 minutes at 80C, snap cool on ice | |||
5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]] |
Revision as of 18:22, 8 July 2013
S2 Probe Prep (Started 5/29/2013)
ULYSIS Dye Coupling (Test)
Going to use the probe Noi gave me a while back to test yield of ULYSIS dye coupling. Going to try two for better confidence.
- Following ULYSIS protocol
1. Add 1 uL DNA in 19 uL labeling buffer (Component C) 2. Denature DNA for 5 minutes at 95C; snap cool on ice 3. Add 1 uL dye to sample; add 4 uL labeling buffer 4. Incubate for 15 minutes at 80C, snap cool on ice 5. Purify using Centri-Sep Column