Daniel:Notebook/HiResChrPaint/2013-7-8: Difference between revisions

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(Created page with "=S2 Probe Prep (Started 5/29/2013)= Back to Calendar ==ULYSIS Dye Coupling (Test)== Going to ...")
 
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  1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
  1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
  3. Denature DNA for 5 minutes at 95C; snap cool on ice
  2. Denature DNA for 5 minutes at 95C; snap cool on ice
  4. Add 1 uL dye to sample; add 4 uL labeling buffer
  3. Add 1 uL dye to sample; add 4 uL labeling buffer
  5. Incubate for 15 minutes at 80C, snap cool on ice
  4. Incubate for 15 minutes at 80C, snap cool on ice
  6. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]]
  5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]]

Revision as of 18:22, 8 July 2013

S2 Probe Prep (Started 5/29/2013)

Back to Calendar

ULYSIS Dye Coupling (Test)

Going to use the probe Noi gave me a while back to test yield of ULYSIS dye coupling.  Going to try two for better confidence.
1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample; add 4 uL labeling buffer
4. Incubate for 15 minutes at 80C, snap cool on ice
5. Purify using Centri-Sep Column