Daniel:Notebook/HiResChrPaint/2013-7-9: Difference between revisions
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>Djacobse (Created page with "=S2 Probe Prep (Started 5/29/2013)= Back to Calendar ==ULYSIS Dye Coupling== Based on [[Danie...") |
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==ULYSIS Dye Coupling== | ==ULYSIS Dye Coupling== | ||
Based on [[Daniel:Notebook/HiResChrPaint/2013-7-8|yesterday's results]] I think it would be helpful to see what happens if I use all 5 uL of dye available (see ULYSIS protocol) in one labeling. | Based on [[Daniel:Notebook/HiResChrPaint/2013-7-8|yesterday's results]] I think it would be helpful to see what happens if I use all 5 uL of dye available (see ULYSIS protocol) | ||
in one labeling. I'll use the oligo Noi gave me again, as I have a lot and it doesn't waste probe. | |||
*Following [[Daniel:Protocols/AlexaDyeCouple#ULYSIS|ULYSIS protocol]] | *Following [[Daniel:Protocols/AlexaDyeCouple#ULYSIS|ULYSIS protocol]] |
Revision as of 17:35, 9 July 2013
S2 Probe Prep (Started 5/29/2013)
ULYSIS Dye Coupling
Based on yesterday's results I think it would be helpful to see what happens if I use all 5 uL of dye available (see ULYSIS protocol) in one labeling. I'll use the oligo Noi gave me again, as I have a lot and it doesn't waste probe.
- Following ULYSIS protocol
1. Add 5 uL DMSO to Alexa dye 488 2. Add 1 uL DNA in 19 uL labeling buffer (Component C) 3. Denature DNA for 5 minutes at 95C; snap cool on ice 4. Add 5 uL dye to sample 5. Incubate for 15 minutes at 80C, snap cool on ice 6. Purify using Centri-Sep Column