Matt:LabNotes/2013-7-11: Difference between revisions

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   Program
   Program
   *98°C 30sec -> (98°C 10sec -> 52°C 30sec -> 72°C 30sec) x8 cycles -> (98°C 10sec -> 72°C 30sec) x16 cycles -> 72°C 3 min -> 15°C hold
   *98°C 30sec -> (98°C 10sec -> 52°C 30sec -> 72°C 30sec) x8 cycles -> (98°C 10sec -> 72°C 30sec) x16 cycles -> 72°C 3 min -> 15°C hold
[[File:071113_PCRAgi20gapProbes_PosCtrl.JPG | 500px]]

Revision as of 23:21, 11 July 2013

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-7-9

Amplification with Sequencing Adapters

Tube # Sample Index#
1 NTC-Agi26k_20gap Indx7
2 gDNA-Agi26k_20gap Indx77
3 cDNA-Agi26k_20gap Indx78
4 gDNA-CES36k18bp Indx48


Components 1X Reaction 5X Reaction
Captured Template 1 0
10uM Forward + Indx 0.4 0
10uM Reverse 0.4 2.0
2X KAPA SYBG FAST MM 12.5 62.5
H2O 10.7 53.5
Total 25 125
 Program
 *98°C 30sec -> (98°C 10sec -> 52°C 30sec -> 72°C 30sec) x8 cycles -> (98°C 10sec -> 72°C 30sec) x16 cycles -> 72°C 3 min -> 15°C hold

File:071113 PCRAgi20gapProbes PosCtrl.JPG