Daniel:Notebook/HiResChrPaint/2013-7-17: Difference between revisions
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1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample for all) | 1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample for all) | ||
{{GelLanes| | {{GelLanes|Lane3=25bp ladder|Lane4=V4S3A}} |
Revision as of 17:27, 17 July 2013
S3 Probe Preparation (Started 7/16/2013)
DpnII Digestion
3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop
DpnII Digest gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp ladder | V4S3A |