Daniel:Notebook/HiResChrPaint/2013-7-17: Difference between revisions

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  3. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], elute with 30 uL EB
  3. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], elute with 30 uL EB
  4. Measure concentrations in nanodrop
  4. Measure concentrations in nanodrop
===Nanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" height="15" | Sample
| width="65" | V4S3A
| width="65" | V4S3B
| width="65" | V4S3C
| width="65" | V4S3D
| width="65" | V6S3A
| width="65" | V6S3B
| width="65" | V6S3C
| width="65" | V6S3D
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 84.9
| align="center" | 82.3
| align="center" | 85.4
| align="center" | 86.4
| align="center" | 107.6
| align="center" | 98.2
| align="center" | 107.4
| align="center" | 109.2
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 30 uL
| align="center" | 2.5
| align="center" | 2.5
| align="center" | 2.6
| align="center" | 2.6
| align="center" | 3.2
| align="center" | 2.9
| align="center" | 3.2
| align="center" | 3.3
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | total ug
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | 10.2
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | 12.7
|}


===DpnII Digest gel===
===DpnII Digest gel===

Revision as of 19:12, 17 July 2013

S3 Probe Preparation (Started 7/16/2013)

Back to Calendar

DpnII Digestion

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

Sample V4S3A V4S3B V4S3C V4S3D V6S3A V6S3B V6S3C V6S3D
ng/uL 84.9 82.3 85.4 86.4 107.6 98.2 107.4 109.2
ug in 30 uL 2.5 2.5 2.6 2.6 3.2 2.9 3.2 3.3
total ug       10.2       12.7

DpnII Digest gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp ladder V4S3A V4S3B V4S3C V4S3D V6S3A V6S3B V6S3C V6S3D
2. Run for 25 minutes at 250V

Gel Image

File:DpnIIDigest 071713.jpg

Lambda Exonuclease Digestion