Hosuk:LabNotesMDA/2013-7-18: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Hosuki78
No edit summary
>Hosuki78
No edit summary
Line 6: Line 6:




====KOH concentration for (ALS+Cell) mix====
====Experiment====
=====KOH concentration for (ALS+Cell) mix=====
*In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''.
*In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''.
*In the v7.3 version, '''269mM''' of KOH in ALS buffer has used ([[Hosuk:LabNotesMDA/2013-7-2|v7.3 ring mixer device]]).
*In the v7.3 version, '''269mM''' of KOH in ALS buffer has used ([[Hosuk:LabNotesMDA/2013-7-2|v7.3 ring mixer device]]).
Line 15: Line 16:




====Experiment====
*Start : 8:30pm 07/17, End : 9:00am 07/18 --> about 13hr reaction
*Start : 8:30pm 07/17, End : 9:00am 07/18 --> about 13hr reaction
*qPCR run for verification
*qPCR run for verification
Line 28: Line 28:




[[File:Backflow_after_opening_valve_2013-07-18.png|600px]]
[[File:Backflow_after_opening_valve_2013-07-18.png|400px]]

Revision as of 18:38, 26 July 2013


MDA with PGP1F, 8th, Dev v9.1

  • Use new device (v9.1)


Experiment

KOH concentration for (ALS+Cell) mix
  • In 20uL reaction, the final concentration of KOH in ALS while denaturing is 240mM.
  • In the v7.3 version, 269mM of KOH in ALS buffer has used (v7.3 ring mixer device).
  • For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).



  • Start : 8:30pm 07/17, End : 9:00am 07/18 --> about 13hr reaction
  • qPCR run for verification


Result
  • Amount of amplicons were good.
  • Some of sample could be lost because of back flow as soon as opening valve
  • So the left 4 chamber were not filled well.
  • I didn't run MDA in ALS chamber, but the reaction will be done in ALS chamber as well for the next experiment.
  • Also I'll try to push the (ALS+Cell) solution into compartment with H2O to prevent sample loss like this.


File:Backflow after opening valve 2013-07-18.png