Hosuk:LabNotesMDA/2013-7-22: Difference between revisions
Jump to navigation
Jump to search
>Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 9th, Dev v9.1=== *Use new device (v9.1) *Same experiment to [[Hosuk:LabNotesMDA/2013-7-18...") |
>Hosuki78 (→Result) |
||
Line 19: | Line 19: | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig1.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-22_fig1.png|400px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig2_Table.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-22_fig2_Table.png|300px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-22_fig3_History.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-22_fig3_History.png|650px]] |
Revision as of 18:51, 26 July 2013
MDA with PGP1F, 9th, Dev v9.1
- Use new device (v9.1)
- Same experiment to 07/18, but try to run MDA in ALS chamber after transferring solution into compartments.
Experiment
- NS valve didn’t work properly but the reaction was kept going. (8hr reaction)
Result
- Not bad, considering bad experiment condition
- Ch25 is the reaction in ALS chamber.
- The amount of amplicon was estimated 483.26pg/uL, which is 724.89pg in total.
- While transferring ALS+Cell solution into compartment with air, the remained solution was kept drying (evaporation by air)
- Thus using H2O would be better than air.