Hosuk:LabNotesMDA/2013-7-26: Difference between revisions
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>Hosuki78 (→Result) |
>Hosuki78 (→Result) |
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**First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air. | **First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air. | ||
**Total amount of DNA estimated were lower than previous run ([[Hosuk:LabNotesMDA/2013-7-18|07/18]]), | **Total amount of DNA estimated were lower than previous run ([[Hosuk:LabNotesMDA/2013-7-18|07/18]]), | ||
**but the distribution of amount of DNAs was | **but the distribution of amount of DNAs was not bad even though no active mixing! | ||
**The samples would have better fragment size because of not much sheer stress. | |||
**It looked enough amount for tagmentation | **It looked enough amount for tagmentation | ||
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*[[File:qPCR_Result_MDAv9.1_2013-07-26_fig1_QGraph_Table.png|700px]] | *[[File:qPCR_Result_MDAv9.1_2013-07-26_fig1_QGraph_Table.png|700px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-26_fig2_History.png|700px]] | *[[File:qPCR_Result_MDAv9.1_2013-07-26_fig2_History.png|700px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-26_fig3_History2.png|700px]] |
Revision as of 19:07, 26 July 2013
MDA with PGP1F, 11th, Dev v9.1
- Use new device (v9.1)
- Same experiment to 07/18, but try pushing ALS+Cell into compartments with H2O, not air.
Experiment
- Start : 5:30pm 07/25, End : 8:30am 07/26 --> about 15hr reaction
- qPCR run for verification
- Extract just about 1.1 uL at each tube and run qPCR.
Result
- Good, considering bad experiment condition
- First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air.
- Total amount of DNA estimated were lower than previous run (07/18),
- but the distribution of amount of DNAs was not bad even though no active mixing!
- The samples would have better fragment size because of not much sheer stress.
- It looked enough amount for tagmentation