Daniel:Notebook/HiResChrPaint/2013-9-4: Difference between revisions

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>Djacobse
(Created page with "=BSPP Control (Started 09/03/2013)= Back to Calendar ==DpnII Digestion ([[Daniel:Notebook/HiResC...")
 
>Djacobse
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===Nanodrop Results===
===Nanodrop Results===


===Gel Image===
===TBE Gel===
 
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)
 
{{GelLanes|Lane4=25bp ladder|Lane5=BSPP 1AB|Lane5=BSPP 1CD|Lane6=BSPP 2AB|Lane7=BSPP 2CD}}
2. Run for 25 minutes at 200V


==Lambda Exonuclease Digestion==
==Lambda Exonuclease Digestion==

Revision as of 18:18, 4 September 2013

BSPP Control (Started 09/03/2013)

Back to Calendar

DpnII Digestion (Started 09/03/2013)

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp ladder BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 25 minutes at 200V

Lambda Exonuclease Digestion