Daniel:Notebook/HiResChrPaint/2013-9-4: Difference between revisions
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>Djacobse (Created page with "=BSPP Control (Started 09/03/2013)= Back to Calendar ==DpnII Digestion ([[Daniel:Notebook/HiResC...") |
>Djacobse |
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===Nanodrop Results=== | ===Nanodrop Results=== | ||
===Gel | ===TBE Gel=== | ||
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all) | |||
{{GelLanes|Lane4=25bp ladder|Lane5=BSPP 1AB|Lane5=BSPP 1CD|Lane6=BSPP 2AB|Lane7=BSPP 2CD}} | |||
2. Run for 25 minutes at 200V | |||
==Lambda Exonuclease Digestion== | ==Lambda Exonuclease Digestion== |
Revision as of 18:18, 4 September 2013
BSPP Control (Started 09/03/2013)
DpnII Digestion (Started 09/03/2013)
3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop
Nanodrop Results
TBE Gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp ladder | BSPP 1CD | BSPP 2AB | BSPP 2CD |
2. Run for 25 minutes at 200V