Blue:RNA-Seq Experiments:09052013: Difference between revisions
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[[File:Cell_Characterization_Worksheet.docx]] | [[File:Cell_Characterization_Worksheet.docx]] | ||
[[File:Data_Submission_Worksheet.docx]] |
Revision as of 23:37, 10 September 2013
Fluidigm C1 Small Chip Beta Test
- Tested NPCs on the small 5-10um chip
- First run on 09-04-2013 produced too few viable cells for loading and resulted in only 10 captured - therefore, reprimed the chip and stored overnight for re-loading
- Second run on 09-05-2013 involved E13.5 neural progenitors sorted as PI negative
- Achieved good loading efficiency (81/96) but high mortality as indicated by live/dead staining on chip
- Followed the protocol exactly - including use of spike-in RNA indicated in the protocol rather than those used in our previous experiments
File:Cell Characterization Worksheet.docx
File:Data Submission Worksheet.docx