Daniel:Notebook/HiResChrPaint/2013-9-11: Difference between revisions
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>Djacobse (Created page with "=Fibroblast Adherence Timecourse (Started 09/09/2013)= Back to Calendar Petri dishes are in! So...") |
>Djacobse No edit summary |
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'''New Template for Experiment''' | '''New Template for Experiment''' | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| width="94" height="30" | Name | |||
| width="65" | Surface | |||
| width="65" | Trypsin Dilution | |||
| width="76" | Cell Seeding Density | |||
| width="88" | Media Kicker | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Slide 09/09/13 | |||
| Slide | |||
| 1:10 | |||
| align="center" | 1.00E+06 | |||
| None | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Slide 1 | |||
| Slide | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| None | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Slide 2 | |||
| Slide | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| 100 uL DMEM | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Dish 1 | |||
| Dish | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| None | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Dish 2 | |||
| Dish | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| 100 uL DMEM | |||
|} |
Revision as of 23:43, 11 September 2013
Fibroblast Adherence Timecourse (Started 09/09/2013)
Petri dishes are in! So I've got a revised itinerary. First, a recap. There are a few important variables to track in this experiment:
- (1) surface type (slide or dish)
- (2) trypsin dilution (to ensure cells may attach)
- (3) Cell seeding density (1,000,000 cells/mL recommended)
- (4) Adding more media to help growth
New Template for Experiment
Name | Surface | Trypsin Dilution | Cell Seeding Density | Media Kicker |
Slide 09/09/13 | Slide | 1:10 | 1.00E+06 | None |
Slide 1 | Slide | 1:20 | 1.00E+06 | None |
Slide 2 | Slide | 1:20 | 1.00E+06 | 100 uL DMEM |
Dish 1 | Dish | 1:20 | 1.00E+06 | None |
Dish 2 | Dish | 1:20 | 1.00E+06 | 100 uL DMEM |