Arichard:Reagents/hmw buffer: Difference between revisions

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>Andrew
(Created page with "From the original patents of Epicentre and Reznikoffs publications, we think the HMW buffer contain the following: Reaction Buffer: (1x Illumina Bf = pH 7.27 @RT, densi...")
 
>Andrew
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Email forwarded from Jeff:
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From the original patents of Epicentre and Reznikoffs publications, we think the HMW buffer contain
From the original patents of Epicentre and Reznikoffs publications, we think the HMW buffer contain
the following:
the following:
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This is likely due to the DMF favors the bending of the DNA to enable the looping of shorter fragments.
This is likely due to the DMF favors the bending of the DNA to enable the looping of shorter fragments.
I hope this is of help.
I hope this is of help.
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End of email.

Latest revision as of 19:22, 13 September 2013

Email forwarded from Jeff:


From the original patents of Epicentre and Reznikoffs publications, we think the HMW buffer contain the following:

Reaction Buffer:      (1x Illumina Bf = pH 7.27 @RT, density=10% DMF) 
5X TA buffer: 165 mM Tris-OAc, pH 7.8, 50 mM Mg-OAc, 330 mM K-OAc, 50% DMF.


From experimenting with the buffer, we obtain longer fragments when lowering the DMF. This is likely due to the DMF favors the bending of the DNA to enable the looping of shorter fragments. I hope this is of help.


End of email.