Arichard:Reagents/als: Difference between revisions

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>Andrew
(Created page with "ALS (Alkaline lysis solution) shelf life is two weeks. To make ALS: 82 ul Nuclease free H2O. 8 ul 5 M KOH. 10 ul 1 M DTT. 2 ul 0.5 M EDTA. KOH shelf life is also two weeks.")
 
>Andrew
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To make ALS:
To make ALS:
82 ul Nuclease free H2O.
*82 ul Nuclease free H2O.
8 ul 5 M KOH.
*8 ul 5 M KOH.
10 ul 1 M DTT.
*10 ul 1 M DTT.
2 ul 0.5 M EDTA.
*2 ul 0.5 M EDTA.


KOH shelf life is also two weeks.
KOH shelf life is also two weeks.

Revision as of 19:23, 13 September 2013

ALS (Alkaline lysis solution) shelf life is two weeks.

To make ALS:

  • 82 ul Nuclease free H2O.
  • 8 ul 5 M KOH.
  • 10 ul 1 M DTT.
  • 2 ul 0.5 M EDTA.

KOH shelf life is also two weeks.