Arichard:Reagents/als: Difference between revisions
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>Andrew (Created page with "ALS (Alkaline lysis solution) shelf life is two weeks. To make ALS: 82 ul Nuclease free H2O. 8 ul 5 M KOH. 10 ul 1 M DTT. 2 ul 0.5 M EDTA. KOH shelf life is also two weeks.") |
>Andrew No edit summary |
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Line 2: | Line 2: | ||
To make ALS: | To make ALS: | ||
82 ul Nuclease free H2O. | *82 ul Nuclease free H2O. | ||
8 ul 5 M KOH. | *8 ul 5 M KOH. | ||
10 ul 1 M DTT. | *10 ul 1 M DTT. | ||
2 ul 0.5 M EDTA. | *2 ul 0.5 M EDTA. | ||
KOH shelf life is also two weeks. | KOH shelf life is also two weeks. |
Revision as of 19:23, 13 September 2013
ALS (Alkaline lysis solution) shelf life is two weeks.
To make ALS:
- 82 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA.
KOH shelf life is also two weeks.