Arichard:Reagents/als: Difference between revisions
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>Andrew No edit summary |
>Andrew No edit summary |
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Line 2: | Line 2: | ||
To make ALS: | To make ALS: | ||
* | * 80 ul Nuclease free H2O. | ||
*8 ul 5 M KOH. | * 8 ul 5 M KOH. | ||
*10 ul 1 M DTT. | * 10 ul 1 M DTT. | ||
*2 ul 0.5 M EDTA. | * 2 ul 0.5 M EDTA. | ||
KOH shelf life is also two weeks. | KOH shelf life is also two weeks. | ||
Alternatively: | |||
* 90 ul 0.11 M DTT | |||
* 8 ul 5 M KOH | |||
* 2 ul EDTA |
Revision as of 20:52, 25 February 2015
ALS (Alkaline lysis solution) shelf life is two weeks.
To make ALS:
- 80 ul Nuclease free H2O.
- 8 ul 5 M KOH.
- 10 ul 1 M DTT.
- 2 ul 0.5 M EDTA.
KOH shelf life is also two weeks.
Alternatively:
- 90 ul 0.11 M DTT
- 8 ul 5 M KOH
- 2 ul EDTA