Arichard:Reagents/als: Difference between revisions

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To make ALS:
To make ALS:
*82 ul Nuclease free H2O.
* 80 ul Nuclease free H2O.
*8 ul 5 M KOH.
* 8 ul 5 M KOH.
*10 ul 1 M DTT.
* 10 ul 1 M DTT.
*2 ul 0.5 M EDTA.
* 2 ul 0.5 M EDTA.


KOH shelf life is also two weeks.
KOH shelf life is also two weeks.
Alternatively:
* 90 ul 0.11 M DTT
* 8 ul 5 M KOH
* 2 ul EDTA

Revision as of 20:52, 25 February 2015

ALS (Alkaline lysis solution) shelf life is two weeks.

To make ALS:

  • 80 ul Nuclease free H2O.
  • 8 ul 5 M KOH.
  • 10 ul 1 M DTT.
  • 2 ul 0.5 M EDTA.

KOH shelf life is also two weeks.

Alternatively:

  • 90 ul 0.11 M DTT
  • 8 ul 5 M KOH
  • 2 ul EDTA