EricChu:LabNotesMDA/2013-9-17: Difference between revisions

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== MDA Device v7 (PGP1 #15) with Tagmentation ==
== MDA Device v7 (PGP1 #15) with Tagmentation ==
* The following samples were labeled as EC-PGP1-Dev7-index#,index#-Sep17 and given to Alan for sequencing.
* They could be pooled into the same lane as they all have different index #s. 5 million reads were asked.


==Size Selection==
==Size Selection==
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|[[File:Sept17TBEgel2cut.jpg|400px]]
|[[File:Sept17TBEgel2cut.jpg|400px]]
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== MDA Device v9 (PGP1 #17) with Tagmentation ==
* Repeating the experiment of 9-15-2013
* Device preparation was described in EricChu:LabNotesMDA/2013-5-20
* MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14
* Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation
* One PGP1 cell was used in this experiment.

Revision as of 21:41, 17 September 2013

MDA Device v7 (PGP1 #15) with Tagmentation

  • The following samples were labeled as EC-PGP1-Dev7-index#,index#-Sep17 and given to Alan for sequencing.
  • They could be pooled into the same lane as they all have different index #s. 5 million reads were asked.

Size Selection

File:Sept17TBEgel1.jpg File:Sept17TBEgel2.jpg
File:Sept17TBEgel1cut.jpg File:Sept17TBEgel2cut.jpg

MDA Device v9 (PGP1 #17) with Tagmentation

  • Repeating the experiment of 9-15-2013
  • Device preparation was described in EricChu:LabNotesMDA/2013-5-20
  • MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14
  • Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation
  • One PGP1 cell was used in this experiment.