Song Chen:Daily/NOTES/2013-9-19: Difference between revisions
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Aliquot Mitomycin C(Roche Cat.#10107409001) | Aliquot Mitomycin C(Roche Cat.#10107409001) | ||
:dissolved in DMEM, 10μg/ml (2 mg in 200 ml) | :dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree | ||
Prepare Mitomycin-C-inactivated MEFs | |||
*... | |||
*3*100 mm MEF(P2) --> 30 vials | |||
*estimated cell density: 3*10<sup>7</sup>(each 100 mm dish) |
Revision as of 00:01, 20 September 2013
Aliquot Mitomycin C(Roche Cat.#10107409001)
- dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree
Prepare Mitomycin-C-inactivated MEFs
- ...
- 3*100 mm MEF(P2) --> 30 vials
- estimated cell density: 3*107(each 100 mm dish)