Song Chen:Daily/NOTES/2013-9-19: Difference between revisions

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Aliquot Mitomycin C(Roche Cat.#10107409001)
Aliquot Mitomycin C(Roche Cat.#10107409001)
:dissolved in DMEM, 10μg/ml (2 mg in 200 ml)
:dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree
 
 
Prepare Mitomycin-C-inactivated MEFs
*...
*3*100 mm MEF(P2) --> 30 vials
*estimated cell density: 3*10<sup>7</sup>(each 100 mm dish)

Revision as of 00:01, 20 September 2013

Aliquot Mitomycin C(Roche Cat.#10107409001)

dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree


Prepare Mitomycin-C-inactivated MEFs

  • ...
  • 3*100 mm MEF(P2) --> 30 vials
  • estimated cell density: 3*107(each 100 mm dish)