Song:MEF Preparation: Difference between revisions
Jump to navigation
Jump to search
>Chensong (Created page with "#Grow passage-3(no more than) primary MEFs to confluency. #Remove the medium, add 10 μg/ml mitomycin-c (diluted in MEF medium) and incubate at 37 °C for 3 h. #Remove the mit...") |
>Chensong No edit summary |
||
Line 3: | Line 3: | ||
#Remove the mitomycin-c solution from the cells and wash them with DPBS for five times. | #Remove the mitomycin-c solution from the cells and wash them with DPBS for five times. | ||
#Trypsinize the cells, collect in MEF medium, and then centrifuge them. | #Trypsinize the cells, collect in MEF medium, and then centrifuge them. | ||
#aliquot into cryovials and freeze at –80 | #aliquot into cryovials and freeze at –80 °C. |
Revision as of 00:18, 20 September 2013
- Grow passage-3(no more than) primary MEFs to confluency.
- Remove the medium, add 10 μg/ml mitomycin-c (diluted in MEF medium) and incubate at 37 °C for 3 h.
- Remove the mitomycin-c solution from the cells and wash them with DPBS for five times.
- Trypsinize the cells, collect in MEF medium, and then centrifuge them.
- aliquot into cryovials and freeze at –80 °C.