AlanFung:LabNotes/Rolony/2013-9-26: Difference between revisions
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==Matlab Image Analysis== |
Revision as of 21:35, 26 September 2013
Strip off dye coupling with formamide
- Aspirate PBS from mattek dish with primary/secondary rolonies
- Add 200ul preheated (65C-80C) 80% formamide in 2X SSC
- Incubate 10min RT
- Rinse w H2O
- Add in 1:200-1uM 200ul (preheat 65C) ACTB-primary rolony target probe (Alexa 488) (200uM ACTB_1stRolonyFISH)
- Incubate 10min RT
- Rinse w 2x SSC twice
- Add in 2X ssc
- image