Song Chen:Daily/NOTES/2013-9-25: Difference between revisions

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*Gelatin coating dishes for HepG2
*Gelatin coating dishes for HepG2
*Alice's HUES: Rack #1 Box #8
*Alice's HUES: Rack #1 Box #8
[[Song:Daily_Notebook|Notebook]]

Revision as of 21:16, 26 September 2013

  • Freezing 293 cells: 1*15 cm--> 8*vials(each for 10 cm dish)
  • Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube
  • Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated)
  • Gelatin coating dishes for HepG2
  • Alice's HUES: Rack #1 Box #8

Notebook