Song Chen:Daily/NOTES/2013-9-25: Difference between revisions
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*Freezing 293 cells: 1*15 cm | *Freezing 293 cells: 1*15 cm → 8*vials(each for 10 cm dish) | ||
*Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube | *Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube | ||
*Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated) | *Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated) |
Latest revision as of 00:10, 3 October 2013
- Freezing 293 cells: 1*15 cm → 8*vials(each for 10 cm dish)
- Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube
- Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated)
- Gelatin coating dishes for HepG2
- Alice's HUES: Rack #1 Box #8