Song Chen:Daily/NOTES/2013-9-25: Difference between revisions

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*Freezing 293 cells: 1*15 cm--> 8*vials(each for 10 cm dish)
*Freezing 293 cells: 1*15 cm → 8*vials(each for 10 cm dish)
*Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube
*Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube
*Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated)  
*Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated)  

Latest revision as of 00:10, 3 October 2013

  • Freezing 293 cells: 1*15 cm → 8*vials(each for 10 cm dish)
  • Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube
  • Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated)
  • Gelatin coating dishes for HepG2
  • Alice's HUES: Rack #1 Box #8


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