Hosuk:LabNotes/2013-9-30: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===1st Rolonies and ACTB Gene detection in 1st Rolonies together=== ====Goal==== *Count ACTB Gene out of 1st rolonies *Analyze how many ACTBs in...") |
>Hosuki78 No edit summary |
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*rehybridize Cy5 labeled probe for 1st rolonies, and ATTO 488 labeled probe for ACTB target | *rehybridize Cy5 labeled probe for 1st rolonies, and ATTO 488 labeled probe for ACTB target | ||
* | * | ||
===PGP1F in 96well plate=== | |||
*For testing multiple sets, cells are split in glass bottom 96 well plate. | |||
*2 well plates are used, and 8 wells in one plate have cells. | |||
====Split condition==== | |||
*About 90% confluenced cells in T25 flask | |||
*After spin dow cells, add 2mL media, | |||
*transfer 60uL cells in a well, and add 100uL media | |||
*Let's see how long it takes time for 100% cells in a well. |
Revision as of 03:14, 1 October 2013
1st Rolonies and ACTB Gene detection in 1st Rolonies together
Goal
- Count ACTB Gene out of 1st rolonies
- Analyze how many ACTBs in all cDNA Rolonies quantitatively
Procedure
- strip previous proves,
- rehybridize Cy5 labeled probe for 1st rolonies, and ATTO 488 labeled probe for ACTB target
PGP1F in 96well plate
- For testing multiple sets, cells are split in glass bottom 96 well plate.
- 2 well plates are used, and 8 wells in one plate have cells.
Split condition
- About 90% confluenced cells in T25 flask
- After spin dow cells, add 2mL media,
- transfer 60uL cells in a well, and add 100uL media
- Let's see how long it takes time for 100% cells in a well.