Daniel:Notebook/HiResChrPaint/2013-9-30: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Fibroblast Culture= Back to Calendar Split passage 9 cells into 3 culture dishes, also prepped 5 plates fo...")
 
>Djacobse
Line 11: Line 11:
  3. Aspirated media and added 1 mL fresh DMEM
  3. Aspirated media and added 1 mL fresh DMEM
  4. Incubated overnight
  4. Incubated overnight
=FISH ([[Daniel:Notebook/HiResChrPaint/2013-9-27|Started 9/27/2013]])=
==Microscopy==
Performed microscopy on samples from 9/27 using Olympus microscope upstairs.
===Results===
'''Control'''
{|class="wikitable"
|-
|[[Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png]]
|-
|40x magnification; BF,DAPI,GFP
|}

Revision as of 19:05, 3 October 2013

Fibroblast Culture

Back to Calendar

Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.

Prepping cells for hybridization

1. Added 100 uL of cell suspension (after step 5) to center of petri dish
2. Incubated for ~9 hours
3. Aspirated media and added 1 mL fresh DMEM
4. Incubated overnight

FISH (Started 9/27/2013)

Microscopy

Performed microscopy on samples from 9/27 using Olympus microscope upstairs.

Results

Control

Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png
40x magnification; BF,DAPI,GFP