Daniel:Notebook/HiResChrPaint/2013-9-30: Difference between revisions
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{|class="wikitable" | {|class="wikitable" | ||
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|[[Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png]] | |[[Image:Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png|800px]] | ||
|- | |- | ||
|40x magnification; BF,DAPI,GFP | |40x magnification; BF,DAPI,GFP | ||
|} | |||
{|class="wikitable" | |||
|- | |||
|[[Image:Dan_exp_overlay_bfdapitxred_10x_FISH_20130930.png|500px]] | |||
|[[Image:Dan_exp_overlay_bfdapitxred_20x_FISH_20130930.png|500px]] | |||
|- | |||
|10x magnification; BF,DAPI,TXRED | |||
|20x magnification; BF,DAPI,TXRED | |||
|- | |||
|[[Image:Dan_exp_overlay_bfdapitxred_40xFISH_20130930.png|500px]] | |||
|[[Image:Overlay-all_FISH_20130930.png|500px]] | |||
|- | |||
|40x magnification; BF,DAPI,TXRED | |||
|40x magnification; BF,DAPI,TXRED,GFP | |||
|} | |} |
Latest revision as of 19:08, 3 October 2013
Fibroblast Culture[edit]
Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.
Prepping cells for hybridization[edit]
1. Added 100 uL of cell suspension (after step 5) to center of petri dish 2. Incubated for ~9 hours 3. Aspirated media and added 1 mL fresh DMEM 4. Incubated overnight
FISH (Started 9/27/2013)[edit]
Microscopy[edit]
Performed microscopy on samples from 9/27 using Olympus microscope upstairs.
Results[edit]
Control
File:Dan exp overlay bfdapigfp 40x FISH 20130930.png |
40x magnification; BF,DAPI,GFP |
File:Dan exp overlay bfdapitxred 10x FISH 20130930.png | File:Dan exp overlay bfdapitxred 20x FISH 20130930.png |
10x magnification; BF,DAPI,TXRED | 20x magnification; BF,DAPI,TXRED |
File:Dan exp overlay bfdapitxred 40xFISH 20130930.png | File:Overlay-all FISH 20130930.png |
40x magnification; BF,DAPI,TXRED | 40x magnification; BF,DAPI,TXRED,GFP |