AlanFung:Protocol/MetaMorph Image Analysis: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 3: | Line 3: | ||
[[File:MetaMorph_Dropin_Commands.pdf]] | [[File:MetaMorph_Dropin_Commands.pdf]] | ||
==Count Nuclei (P.789 in Dropin Commands)== | ==Count Nuclei (P.789 in Dropin Commands)== | ||
*Use this application module to segment images that are used to identify and differentiate cell nuclei. | |||
*The segmentation labels each isolated and identified nucleus as a different color index to enable you to see a visual separation between nuclei that are close or touching. | |||
*'''Source images must be 16-bit images.''' | |||
Three settings are available to you: | |||
*Approximate minimum nuclei width, Approximate maximum nuclei width, and the minimum gray level intensity of the nuclei above the local background. | |||
*Any nuclei-like pattern in the image less than the specified minimum width will be considered to be noise, and will be excluded from the segmentation. | |||
===Parameters=== | ===Parameters=== | ||
===Determine Dimensions=== | ===Determine Dimensions=== | ||
===Determine Intensity above local background=== | ===Determine Intensity above local background=== |
Revision as of 23:47, 16 October 2013
Guide
File:MetaMorph Basic Commands.pdf File:MetaMorph Dropin Commands.pdf
Count Nuclei (P.789 in Dropin Commands)
- Use this application module to segment images that are used to identify and differentiate cell nuclei.
- The segmentation labels each isolated and identified nucleus as a different color index to enable you to see a visual separation between nuclei that are close or touching.
- Source images must be 16-bit images.
Three settings are available to you:
- Approximate minimum nuclei width, Approximate maximum nuclei width, and the minimum gray level intensity of the nuclei above the local background.
- Any nuclei-like pattern in the image less than the specified minimum width will be considered to be noise, and will be excluded from the segmentation.