Daniel:Notebook/HiResChrPaint/2013-10-23: Difference between revisions

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(Created page with "=BSPP Control (Started 10/22/2013)= Back to Calendar ==DpnII Digestion ([[Daniel:Notebook/HiRe...")
 
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===Nanodrop Results===
===Nanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" height="15" | Sample
| width="65" | BSPP 1AB
| width="65" | BSPP 1CD
| width="65" | BSPP 2AB
| width="65" | BSPP 2CD
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 166.5
| align="center" | 168.8
| align="center" | 180.2
| align="center" | 143
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 30 uL
| align="center" | 5.0
| align="center" | 5.1
| align="center" | 5.4
| align="center" | 4.3
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | total
| align="center" | &nbsp;
| align="center" | 10.1
| align="center" | &nbsp;
| align="center" | 9.7
|}


===TBE Gel===
===TBE Gel===

Revision as of 18:40, 23 October 2013

BSPP Control (Started 10/22/2013)

Back to Calendar

DpnII Digestion (Started 10/22/2013

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

Sample BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
ng/uL 166.5 168.8 180.2 143
ug in 30 uL 5.0 5.1 5.4 4.3
total   10.1   9.7

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7.2 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 10bp ladder BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 23 minutes at 220V
3. Stain with 3 uL SYBR gold for 2 minutes

Gel Image