Arichard:Reagents/nexteraDilution: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Andrew
(Created page with "From Shendure protocol in Jeff's notes: 1 μl Nextera enzyme + 24 μl TE + 25 μl 100% glycerol. It is easier, however, to make a large solution of TE/glycerol to avoid pipet...")
 
>Andrew
No edit summary
 
Line 1: Line 1:
UV treat TE and glycerol before adding enzyme.
From Shendure protocol in Jeff's notes: 1 μl Nextera enzyme + 24 μl TE + 25 μl 100% glycerol.
From Shendure protocol in Jeff's notes: 1 μl Nextera enzyme + 24 μl TE + 25 μl 100% glycerol.


It is easier, however, to make a large solution of TE/glycerol to avoid pipeting small volumes of glycerol.
It is easier, however, to make a large solution of TE/glycerol to avoid pipeting small volumes of glycerol.


For example, mix 2.4 ml TE + 25 ml 100% glycerol --> 49 ul of this solution + 1 ul of Nextera = 50 ul 1:50 dilution.
For example, mix 2.4 ml TE + 2.5 ml 100% glycerol --> 49 ul of this solution + 1 ul of Nextera = 50 ul 1:50 dilution.

Latest revision as of 17:17, 6 March 2014

UV treat TE and glycerol before adding enzyme.

From Shendure protocol in Jeff's notes: 1 μl Nextera enzyme + 24 μl TE + 25 μl 100% glycerol.

It is easier, however, to make a large solution of TE/glycerol to avoid pipeting small volumes of glycerol.

For example, mix 2.4 ml TE + 2.5 ml 100% glycerol --> 49 ul of this solution + 1 ul of Nextera = 50 ul 1:50 dilution.