AlanFung:LabNotes/Probe/2013-12-11: Difference between revisions
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*Removal of amplification adapters | *Removal of amplification adapters | ||
*Probe purification by PAGE size-selection | *Probe purification by PAGE size-selection | ||
==Expansion PCR== | |||
*Probe concentration from CustomArray 32.71ng/ul volume 80uL Amount 2.62ug |
Revision as of 20:00, 11 December 2013
GP1 Padlock Probe Production
Overview
- Expansion PCR
- Production PCR
- Exonuclease digestion to generate SS-DNA
- Removal of amplification adapters
- Probe purification by PAGE size-selection
Expansion PCR
- Probe concentration from CustomArray 32.71ng/ul volume 80uL Amount 2.62ug