AlanFung:LabNotes/Probe/2013-12-11: Difference between revisions

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==Expansion PCR==
==Expansion PCR==
*Probe concentration from CustomArray  32.71ng/ul volume 80uL Amount 2.62ug
*Probe concentration from CustomArray  32.71ng/ul volume 80uL Amount 2.62ug
===GP1_V4 Expansion PCR===
===GP1_V6 Expansion PCR===

Revision as of 20:03, 11 December 2013

GP1 Padlock Probe Production

Overview

  • Expansion PCR
  • Production PCR
  • Exonuclease digestion to generate SS-DNA
  • Removal of amplification adapters
  • Probe purification by PAGE size-selection

Expansion PCR

  • Probe concentration from CustomArray 32.71ng/ul volume 80uL Amount 2.62ug

GP1_V4 Expansion PCR

GP1_V6 Expansion PCR