AlanFung:Protocol/EZ DNA Methylation-Direct: Difference between revisions
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*Sample Digestion with Proteinase K | *Sample Digestion with Proteinase K | ||
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| align="center" style="background:#f0f0f0;"| | | align="center" style="background:#f0f0f0;"| | ||
| align="center" style="background:#f0f0f0;"|'''2000 cells''' | | align="center" style="background:#f0f0f0;"|'''2000 cells''' | ||
| align="center" style="background:#f0f0f0;"|'''100,000 cells''' | | align="center" style="background:#f0f0f0;"|'''100,000 cells''' |
Revision as of 20:33, 14 August 2009
Draft
EZ_DNA_Methylation_Direct_Kit_Protocol PDF
Overview
- Sample preparation
- Sample Digestion with Proteinase K
- Bisulfite Conversion of DNA
Protocol
- Sample Preparation
- Adherent cells
- Suspension cells
- Sample Digestion with Proteinase K
2000 cells | 100,000 cells | |
M-Digestion Buffer | 10ul | 13ul |
Sample | up to 9ul | up to 12ul |
Proteinase K | 1ul | 1ul |
H20 | X ul | x ul |
Total Volume | 20ul | 26 ul |