AlanFung:Protocol/EZ DNA Methylation-Direct: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
No edit summary
>Alan6017518
Line 17: Line 17:
*Sample Digestion with Proteinase K
*Sample Digestion with Proteinase K
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|
| align="center" style="background:#f0f0f0;"|'''2000 cells'''
| align="center" style="background:#f0f0f0;"|'''2000 cells'''
| align="center" style="background:#f0f0f0;"|'''100,000 cells'''
| align="center" style="background:#f0f0f0;"|'''100,000 cells'''

Revision as of 20:33, 14 August 2009

Draft

EZ_DNA_Methylation_Direct_Kit_Protocol PDF

Overview

  • Sample preparation
  • Sample Digestion with Proteinase K
  • Bisulfite Conversion of DNA

Protocol

  • Sample Preparation
  • Adherent cells
  • Suspension cells
  • Sample Digestion with Proteinase K
2000 cells 100,000 cells
M-Digestion Buffer 10ul 13ul
Sample up to 9ul up to 12ul
Proteinase K 1ul 1ul
H20 X ul x ul
Total Volume 20ul 26 ul