Daniel:Notebook/HiResChrPaint/2014-1-9: Difference between revisions
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>Djacobse (Created page with "=Alu Control (Started 1/08/2014)= Back to Calendar ==DpnII Digestion== I'm going to do a test d...") |
>Djacobse |
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==DpnII Digestion== | ==DpnII Digestion== | ||
I'm going to do a test digestion/gel to make sure this works. | I'm going to do a test digestion/gel to make sure this works. Since I had such low input I'm going to use 2 samples and pool after the digestion. | ||
1. Reaction Table | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| width="65" height="15" | Sample | |||
| width="65" | ALU2-A | |||
| width="65" | ALU2-B | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ng/uL | |||
| align="center" | 25.1 | |||
| align="center" | 25 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug in 30 uL | |||
| align="center" | 0.8 | |||
| align="center" | 0.8 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="30" | uL 10X buffer | |||
| align="center" | 5 | |||
| align="center" | 5 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="30" | uL DpnII (50U/uL) | |||
| align="center" | 1 | |||
| align="center" | 1 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | uL nfH2O | |||
| align="center" | 14 | |||
| align="center" | 14 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Total | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
|} | |||
2. Incubate samples at 37C for '''2''' hours; 20 minute heat shock at 65C | |||
3. Column Purification, elute with 30 uL EB | |||
4. Measure concentrations in nanodrop |
Revision as of 17:52, 9 January 2014
Alu Control (Started 1/08/2014)
DpnII Digestion
I'm going to do a test digestion/gel to make sure this works. Since I had such low input I'm going to use 2 samples and pool after the digestion.
1. Reaction Table
Sample | ALU2-A | ALU2-B |
ng/uL | 25.1 | 25 |
ug in 30 uL | 0.8 | 0.8 |
uL 10X buffer | 5 | 5 |
uL DpnII (50U/uL) | 1 | 1 |
uL nfH2O | 14 | 14 |
Total | 50 | 50 |
2. Incubate samples at 37C for 2 hours; 20 minute heat shock at 65C 3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop