Daniel:Notebook/HiResChrPaint/2014-1-9: Difference between revisions

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>Djacobse
(Created page with "=Alu Control (Started 1/08/2014)= Back to Calendar ==DpnII Digestion== I'm going to do a test d...")
 
>Djacobse
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==DpnII Digestion==
==DpnII Digestion==


I'm going to do a test digestion/gel to make sure this works.
I'm going to do a test digestion/gel to make sure this works. Since I had such low input I'm going to use 2 samples and pool after the digestion.
 
1. Reaction Table
 
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" height="15" | Sample
| width="65" | ALU2-A
| width="65" | ALU2-B
 
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 25.1
| align="center" | 25
 
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 30 uL
| align="center" | 0.8
| align="center" | 0.8
 
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="30" | uL 10X buffer
| align="center" | 5
| align="center" | 5
 
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="30" | uL DpnII (50U/uL)
| align="center" | 1
| align="center" | 1
 
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | uL nfH2O
| align="center" | 14
| align="center" | 14
 
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | Total
| align="center" | 50
| align="center" | 50
 
|}
 
2. Incubate samples at 37C for '''2''' hours; 20 minute heat shock at 65C
3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Revision as of 17:52, 9 January 2014

Alu Control (Started 1/08/2014)

Back to Calendar

DpnII Digestion

I'm going to do a test digestion/gel to make sure this works. Since I had such low input I'm going to use 2 samples and pool after the digestion.

1. Reaction Table
Sample ALU2-A ALU2-B
ng/uL 25.1 25
ug in 30 uL 0.8 0.8
uL 10X buffer 5 5
uL DpnII (50U/uL) 1 1
uL nfH2O 14 14
Total 50 50
2. Incubate samples at 37C for 2 hours; 20 minute heat shock at 65C
3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop