Hosuk:LabNotes/2014-1-9: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== *PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer...") |
>Hosuki78 No edit summary |
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*Overlap count of RAB7A : count = 14 | *Overlap count of RAB7A : count = 14 | ||
**''''Almost no RAB7A target in 1st Rolony…WHY?…''' | **''''Almost no RAB7A target in 1st Rolony…WHY?…''' | ||
*Raw images | |||
**[[Media:MIP_RTPrimer_RAB7A-2_Step2_1stRolony-Cy5.tif|D1000, 1st Rolony]] | |||
**[[Media:MIP_RTPrimer_RAB7A-2_Step1_RAB7A-ATTO550.tif|D1000, RAB7A target detection]] | |||
*[[File:MIP_RTPrimer_1stRolony_RAB7A_detection.png|800px]] |
Revision as of 18:40, 10 January 2014
RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish
- PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
Result
- 1st Rolony detection probe (Cy5) : count = 5135
- RAB7A detection probe (ATTO550) : count = 64
- Overlap count of RAB7A : count = 14
- 'Almost no RAB7A target in 1st Rolony…WHY?…